Genetic Diversity of Philippine Allium Sativum L. (Alliaceae) Using Random Amplified Polymorphic Dna (Rapd) Analysis
Philippine Journals Online
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| Title |
Genetic Diversity of Philippine Allium Sativum L. (Alliaceae) Using Random Amplified Polymorphic Dna (Rapd) Analysis
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| Creator |
Vivian A. Panes; Department of Biology, Ateneo de Manila University
Ralph Arnold S. Lasala; Department of Biology, Ateneo de Manila University Vivian S. Tolentino; Department of Biology, Ateneo de Manila University Merab A. Chan; Department of Biology, Ateneo de Manila University |
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| Subject |
Science; Engineering; Biology
genetic diversity; RAPD; garlic; Allium sativum L.; Alliaceae |
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| Description |
Allium sativum L., commonly known as garlic, is an important and widely cultivated plant with both culinary and medicinal uses. In this study, morphological data such as bulb size, bulb weight, number of scales, number of cloves, weight of cloves and growth rate of garlic samples from ten different sites in the Philippines were collected. Genomic DNA was extracted and subsequent random amplified polymorphic DNA (RAPD) analysis was performed. Genetic diversity and distances were calculated using Nei’s (1979) index of similarity. Dendrograms were constructed using the NTSYS-pc software version 2.1, a software that computes for the genetic similarity values and Unweighted Pair Group Method with Arithmetic Mean (UPGMA) to construct the dendrogram based on the similarity coefficient matrices. Results of the morphological data analysis based on ANOVA showed that the six morphological features are significantly different at .05 level of significance. RAPD yielded a total of 69 bands from eight RAPD primers, ranging from 6 to 15 bands, corresponding to a mean of 8.6 bands per primer. The total polymorphic loci for all the sixteen A. sativum accessions is 78.3% which is fairly high. The genetic variation ranged from 0.1107 to 0.4983 with a mean of 0.2532. These values are as well fairly high. Dendrograms constructed using the NTSYS-pc program produced 6 clusters. Similarity coefficients are between 24%-75% only, which means that A. sativum from the ten sampling sites are genetically diverse. Allium ascalonicum which is an outgroup Panes, Lasala, Tolentino, & Chan diverged from all the A. sativum accessions in the dendrogram which confirmed that it is indeed an outgroup. This study has proven that RAPD is a useful DNA marker technology in determining genetic diversity among the various accessions of A. sativum collected from 10 sampling sites in the Philippines.
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| Publisher |
Loyola Schools, Ateneo de Manila University
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| Contributor |
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| Date |
2008-05-22
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| Type |
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| Format |
application/pdf
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| Identifier |
http://www.philjol.info/index.php/LSR/article/view/288
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| Source |
Loyola Schools Review; Vol 6 (2007); 43-63
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| Language |
en
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| Coverage |
Philippines
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| Rights |
The individual authors assert the copyright of their works, and the Office of Research and Publications asserts the copyright of the journals under the Intellectual Property Code of the Philippines [Republic Act No. 8293] and any provisions thereof. For inquiries on multiple reproduction or publication of the works, please contact orp@admu.edu.ph
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