Penentuan Waktu Ekuilibrasi pada Pembekuan Semen Kuda Menggunakan Bahan Pengencer Susu Skim
Indonesian Journals Online
View Archive Info| Field | Value | |
| Title |
Penentuan Waktu Ekuilibrasi pada Pembekuan Semen Kuda Menggunakan Bahan Pengencer Susu Skim
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| Creator |
Raden Iis Arifiantini
Iman Supriatna Samsurizal Samsurizal |
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| Subject |
Equilibration, frozen semen, stallion
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| Description |
The objectives of this experiment were to determine the best equilibration time with extender skim milk in
order to maintain the quality of frozen stallion semen. The semen was collected from three 5-8 years old stallions using
artificial vagina. The semen characteristics and quality were evaluated macro- and microscopically and extended with skim
milk extender (1:1). The semen was centrifuged at 3000 rpm for 15 minutes. Supernatant was removed and the pellet was
re-extended with skim milk extender with 5% glycerol. The extended semen then packed in mini straw (0.5 mL),
equilibrated at 5oC for 1 hr (E1), 2 hrs (E2) and 3 hrs (E3), frozen in the liquid nitrogen (N2) vapor for 15 minutes and then
stored in liquid N2 container for 24 hrs. The straw than thawed at 37oC for 30 seconds. The percentages of sperm motility
and viability were observed. The result of this research showed that there were no significant differences between
equilibration at 1, 2 and 3 hrs on the percentages of sperm motility with the average of post thawed motility was 23.60 ±
7.10%. The equilibration time affected the sperm viability (P
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| Publisher |
—
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| Contributor |
—
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| Date |
2008-04-05
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| Type |
Peer-reviewed Article
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| Format |
application/pdf
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| Identifier |
http://www.ijonline.net/index.php/JAP/article/view/225
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| Source |
Animal Production, Jurnal Produksi Ternak; Vol 9, No 3 (2007)
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| Language |
en
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