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| Field |
Value |
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| Title |
Ekstraksi Kolagen Cakar Ayam dengan Berbagai Jenis Larutan dan Lama Perendaman
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| Creator |
Prayitno Prayitno
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| Subject |
Chicken feet, acids, soaking time, collagen
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| Description |
The objective of this research was to know the ability of various acidic solutions on dissolving collagenchicken feet, with different soaked time. Each acid 5 percent (v/v), collagen extraction was done by washing chicken feetand then cutted into small pieces and finally grinded. Every 100 gram treatment was soaked in acetic acid (a1), citric acid(a2), lactic acid (a3) and hydrochloric acid (a4), for 12, 24 and 36 hours. Precipitated collagen in the filtrate was 5 percentNaOH to reach the neutral pH (pH 7). Collagen precipitate was separated by filtration usingfilter paper and thenrendement was calculated, HPLC was used to determin amino acid composition, and SDS-PAGE was use determin the typeof collagen. This experiment use factorial completely randomized design (CRD) 4 x 3 and three time replication. Resultshowed that lactic acid has highest capability to dissolve collagen, while citric acid the lowest. Combination of acidsolution and soaking time had significant (P
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| Publisher |
—
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| Contributor |
—
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| Date |
2008-01-20
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| Type |
Peer-reviewed Article
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| Format |
application/pdf
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| Identifier |
http://www.ijonline.net/index.php/JAP/article/view/116
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| Source |
Animal Production, Jurnal Produksi Ternak; Vol 9, No 2 (2007)
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| Language |
en
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