STUDI AKTIVITAS ENZIM PROTEASE EKSTRASELULER STREPTOKOKUS GRUP B (Streptococcus agalactiae) PADA SUBSTRAT MUSIN
Indonesian Journals Online
View Archive Info| Field | Value | |
| Title |
STUDI AKTIVITAS ENZIM PROTEASE EKSTRASELULER STREPTOKOKUS GRUP B (Streptococcus agalactiae) PADA SUBSTRAT MUSIN
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| Creator |
Achmad Slamet Aku
Muhammad Amrullah Pagala |
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| Subject |
protease, SGB, mucin, enzym characterization, purification.
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| Description |
Group B Streptococci (GBS) produce extracellular enzymes which are know to be involved invirulence mechanism. One of the enzymes is protease which has an ability to degradate mucin. Mucin hasan important role to defend mucosal membrane from invasion of bacteria. The purpose of this researchwas to analysis protease characteristics of GBS on the mucin substrat as a model for studying the role ofGBS proteases in pathogenesis of GBS infections. The optimum pH for the enzym was 7 and the enzymehad maximal activity at 370 C. The protease activity was inhibited by EDTA (2 mM and 5 mM) and PMSF(2 mM and 5 mM). The protease activity was enhanced by addition of MgCl2, CaCl2, and Fe2Cl3 (2 mMand 5 mM).
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| Publisher |
Agriplus
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| Contributor |
—
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| Date |
2008-01-03
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| Type |
Peer-reviewed Article
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| Format |
application/pdf
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| Identifier |
http://www.ijonline.net/index.php/Agriplus/article/view/126
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| Source |
Agriplus; Vol 17, No 2 (2007)
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| Language |
en
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