Deteksi Cepat Penyakit Pustul Bakteri pada Kedelai Menggunakan Teknik PCR dengan Primer Spesifik
Indonesian Journals Online
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| Title |
Deteksi Cepat Penyakit Pustul Bakteri pada Kedelai Menggunakan Teknik PCR dengan Primer Spesifik
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| Creator |
ANDI KHAERUNI
ANTONIUS SUWANTO BUDI JAHJONO MEITY SURADJI SINAGA |
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| Subject |
Xanthomonas axonopodis pv. glycines, bacterial pustule disease, rapid detection, PCR, specific primer
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| Description |
A rapid polymerase chain reaction (PCR)-based procedure was developed for detection of Xanthomonas axonopodis pv.
glycines, the causal agent of bacterial pustule disease on soybean. A set of primers was designed from partial sequence of
the pathogenicity gene of X. axonopodis pv. glycines strain YR32. Specific PCR product of 490 base pairs was produced from
strains of X. axonopodis pv. glycines originally from Indonesia as well as from Taiwan. No other pathovars and bacterial species
among those tested showed amplification product under optimized PCR conditions. Shaking infected soybean leaves in
phosphate buffer saline during six hours was proved to be an essential in order to increase cell number of the bacterial. The
procedure was applicable and reliable for detecting of pathogens in infected plant materials. The procedure was proved to
be more effective than that of conventional detection and could be of great help for monitoring of pustule bacterial
disease in the soybean fields.
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| Publisher |
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| Date |
2008-01-25
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| Type |
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| Format |
application/pdf
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| Identifier |
http://www.ijonline.net/index.php/HAYATI/article/view/152
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| Source |
HAYATI, Journal of Biosciences; Vol 14, No 2 (2007)
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| Language |
en
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