In Vitro Selection of Abaca for Resistance to Fusarium oxysporum f.sp. cubense
Indonesian Journals Online
View Archive Info| Field | Value | |
| Title |
In Vitro Selection of Abaca for Resistance to Fusarium oxysporum f.sp. cubense
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| Creator |
RULLY DYAH PURWATI
SAID HARRAN SUDARSONO SUDARSONO |
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| Subject |
Fusarium wilt, in vitro selection, culture filtrate, ethylmethanesulphonate (EMS), Musa textilis Nee
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| Description |
Abaca (Musa textilis Nee) is an important industrial crop. However, the cultivation of this crop in Indonesia is hampered byFusarium wilt (Panama disease) as a result of Fusarium oxysporum f.sp. cubense (Foc) infection. The objectives of thisstudy were to (i) evaluate inhibitory effects of culture filtrates (CF) of three Foc isolates (Banyuwangi, Malang, andBojonegoro isolates) on shoot growth of abaca cv. Tangongon and Sangihe-1, (ii) determine sublethal concentration of FocCF, (iii) isolate variant cells/tissues which are insensitive against Foc CF and regenerate plantlets from the variants, and(iv) evaluate responses of the plantlets against Foc infection. The results of the experiment showed that even though CFof all Foc isolates inhibited abaca’s shoot growth, CF of Foc Banyuwangi isolate showed the most inhibitory effect.Sublethal concentration of CF of Foc Banyuwangi isolate was 40%. From abaca cv. Tangongon, 326 shoots wereregenerated from CF insensitive embryogenic calli while from Sangihe-1 - 176 shoots were regenerated. Followingacclimatization and Foc inoculation using detached-leaf dual culture test, a total of four immune, two resistant, and twomoderately resistant plantlets were identified out of 45 tested variants of Tangongon. On the other hand, only tworesistant and one moderately resistant plantlets were identified out of 10 tested variants of Sangihe-1.
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| Publisher |
—
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—
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| Date |
2008-01-25
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| Type |
Peer-reviewed Article
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| Format |
application/pdf
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| Identifier |
http://www.ijonline.net/index.php/HAYATI/article/view/150
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| Source |
HAYATI, Journal of Biosciences; Vol 14, No 2 (2007)
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| Language |
en
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