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| Field |
Value |
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| Title |
Direct Cloning of a Xylanase Gene from Pawan-Riau Hot Spring
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| Creator |
IS HELIANTI
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| Subject |
β-1, 4-endoxylanase, metagenomic DNA, Pawan-Riau hot-spring
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| Description |
A functional gene containing an Open Reading Frame (ORF) encoding a β-1, 4-endoxylanase glycosyl hydrolasefamily 11 was cloned directly using metagenomic PCR-cloning method from Pawan Hot Spring sample in Riau. The geneconsisted of 642 nucleotides, encoded for 213 amino acids. The amino acid sequence analysis using BLAST showed thatthe gene has high homology (93%) with xylanase gene from Bacillus subtilis. The gene showed its function when it wassubcloned into an expression vector and overexpressed in E. coli. The crude extract of the recombinant enzyme hadactivity for 170 U/ml at 50 oC. The result of this work showed that metagenomic approach was a powerful short cut methodto obtain recombinant biocatalyst that was useful for industrial application.
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| Publisher |
—
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| Contributor |
—
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| Date |
2008-01-25
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| Type |
Peer-reviewed Article
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| Format |
application/pdf
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| Identifier |
http://www.ijonline.net/index.php/HAYATI/article/view/148
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| Source |
HAYATI, Journal of Biosciences; Vol 14, No 2 (2007)
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| Language |
en
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