Micropropagation of Eclipta alba (Linn.) Hassk- a Valuable Medicinal Herb
Bangladesh Journals Online
View Archive Info| Field | Value | |
| Title |
Micropropagation of Eclipta alba (Linn.) Hassk- a Valuable Medicinal Herb
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| Creator |
AKM Sayeed Hassan; Biological Research Division, BCSIR Laboratories, Dhaka-1205, Bangladesh
Farhana Afroz; Biological Research Division, BCSIR Laboratories, Dhaka-1205, Bangladesh Laila Shamroze Bari; Biological Research Division, BCSIR Laboratories, Dhaka-1205, Bangladesh John Liton Munshi; Biological Research Division, BCSIR Laboratories, Dhaka-1205, Bangladesh Miskat Ara Akhter Jahan; Biological Research Division, BCSIR Laboratories, Dhaka-1205, Bangladesh Rahima Khatun; Biological Research Division, BCSIR Laboratories, Dhaka-1205, Bangladesh |
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| Subject |
Applied Science
Eclipta alba; Medicinal plant; Shoot proliferation; Micropropagation; Acclimatization — |
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| Description |
A protocol was established for mass propagation of a valuable medicinal herb, Eclipta alba (Linn.) Hassk (Asteraceae) through in vitro culture. Apical and axillary buds of young sprouts from selected plants were used as explants. Best shoot induction was observed on MS basal medium supplemented with 0.5 mgl-1 BAP + 0.1 mgl-1 NAA, in which 94% of the explants produced 18 shoots per culture. Repeated subcultures in the same medium, resulted rapid shoot multiplication with 26 shoots per culture. In vitro raised shoots rooted on half strength MS medium with 1.0 mgl-1 IBA +1.0 mgl-1 NAA. For acclimatization and transplantation, the plantlets in the rooting culture tubes were kept in normal room temperature for 7 days before transplanting in pots where plantlets were reared for three weeks. The survival rate of regenerated plantlets was 80%. Key words: Eclipta alba, Medicinal plant, Shoot proliferation, Micropropagation, Acclimatization DOI: 10.3329/bjsir.v43i2.965 Bangladesh J. Sci. Ind. Res. 43(2), 215-222, 2008
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| Publisher |
Bangladesh Council of Scientific & Industrial Research
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| Date |
2008-07-09
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| Format |
application/pdf
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| Identifier |
http://www.banglajol.info/index.php/BJSIR/article/view/965
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| Source |
Bangladesh Journal of Scientific and Industrial Research; Vol 43, No 2 (2008); 215-222
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| Language |
en
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