A Simple RP-HPLC Method for the Determination of Omeprazole in Human Serum and Urine: Validation and Application in Pharmacokinetic Study
Bangladesh Journals Online
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| Title |
A Simple RP-HPLC Method for the Determination of Omeprazole in Human Serum and Urine: Validation and Application in Pharmacokinetic Study
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| Creator |
Kamrun Nahar; Department of Pharmacy, University of Asia Pacific, Dhanmondi, Dhaka
Jafreen Jamal Joti; Department of Pharmacy, University of Asia Pacific, Dhanmondi, Dhaka Md Ashik Ullah; Department of Clinical Pharmacy and Pharmacology, University of Dhaka, Dhaka Ahasanul Hasan; Department of Clinical Pharmacy and Pharmacology, University of Dhaka, Dhaka Mohammad Abul Kalam Azad; Department of Pharmaceutical Technology, University of Dhaka, Dhaka Abul Hasnat; Department of Clinical Pharmacy and Pharmacology, University of Dhaka, Dhaka |
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| Subject |
Pharmacy
Omeprazole; RP-HPLC; Method validation — |
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| Description |
A Simple RP-HPLC method with UV detection has been validated to determine omeprazole concentrations in human serum and urine samples. The mobile phase consisted of a mixture of potassium dihydrogen phosphate buffer (pH 7.2 ± 0.05; 0.2 M) and acetonitrile (70:30, v/v), pumped at a flow rate of 1.0 ml/min through the C-8 column at room temperature. Peaks were monitored by UV absorbance at 302 nm at a sensitivity of 0.0001. The developed method was selective and linear for omeprazole concentrations ranging between 5 to 1000ng/ml for serum samples and 1 to 100μg/ml for urine samples. The recovery of omeprazole ranged from 95.68 to 99% and 95.54 to 99.8% for the serum and urine samples respectively. The limit of quantitation (LOQ) of omeprazole was 5 ng/ml. The intraday accuracy ranged from 93.54 to 104.38% and 100.55 to 103.48% for the serum and urine respectively. The interday accuracy varied from 97.61 to 113.95% and 97.42 to 109.97% for the serum and urine respectively. For the LOQ, good values of precision (6.03 and 10.13% for intraday and interday, respectively) were also obtained. Acceptable results were obtained during stability study. This method proved to be simple, accurate and precise for pharmacokinetic and bioequivalence studies of omeprazole. Key words: Omeprazole; RP-HPLC; Method validation. DOI: 10.3329/dujps.v8i2.6026 Dhaka Univ. J. Pharm. Sci. 8(2): 123-130, 2009 (December)
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| Publisher |
Dhaka University
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| Contributor |
—
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| Date |
2010-09-06
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| Type |
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| Format |
application/pdf
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| Identifier |
http://www.banglajol.info/index.php/JPharma/article/view/6026
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| Source |
Dhaka University Journal of Pharmaceutical Sciences; Vol 8, No 2 (2009); 123-130
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| Language |
en
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| Coverage |
Bangladesh
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